This is the first machine that is designed to icroencapsulate cells in alginate membrane leading to the growth of microtissues or 3D cells. This product has the advantage of allowing exchange of media and produced uniform size of microtissues. The size control is based on the optimized flow rate of alginate and flicking speed.
With this machine, 80 pieces per minute of microcapsules can be produced. The quantity of the microtissues produces is unlimited as long as cells are available. The existing product in the market are such as HydroMatrix™ Peptide Hydrogel, 3D Petri Dish®, GravityPLUS™, NanoShuttleTM-PL that are more of polymer based micromolds that enable cells to aggregate in microwells. The difficulty in controlling the consistency in depositing cells in each microwell through pipetting.
This is the first machine that produce 3D cells that can be used long term. Battery operated and lower power consumption. Established use of alginate (FDA approved biomaterial) for microencapsulation at narrow distribution of 200-300 µm. Controllable flow rate to increase size of microcapsules up to 450 µm. Simple operation and the user can be trained to operate the machine in 15 minutes. Produce high yield of microtissues (80 pieces/min) and control density of cells.
Pintors (Location Identifier created to identify location of scholarly research project online)
|Soon, C. F. (2018). Flicking System For Producing High Throughput 3D Cells. Bangi, Selangor, Malaysia: MTUN Research Repository. QOI: 60.43650.2018-MTUNConnect.N037167|